Efeito da prostaglandina F2α no início do protocolo de sincronização da ovulação em novilhas leiteiras Bos indicus, Bos taurus e Bos indicus x Bos taurus

J. B. P. Carvalho, N. A. T. Carvalho, E. L. Reis, M. Nichi, J. G. Soares, P. S. Baruselli

Resumo


O objetivo do presente estudo foi avaliar o efeito do tratamento com prostaglandina F2α (PGF2α) no início do protocolo de sincronização da ovulação, sobre a dinâmica folicular de novilhas Bos indicus (Gir; n=11), Bos taurus (Holandesa Preto e Branco - HPB; n=10) e cruzadas (Gir x HPB; n=12). Em dia aleatório do ciclo estral (dia 0 - D0), os animais receberam 2,0 mg de benzoato de estradiol (BE) via intramuscular, mais um dispositivo intravaginal de progesterona (P4) que foi mantido por oito dias (dia 8 – D8). Metade das novilhas de cada grupo recebeu uma dose de 25 mg de PGF2α via intramuscular no momento da inserção do dispositivo intravaginal de P4. Na retirada do dispositivo intravaginal (D8), todos os animais receberam outra dose de 25 mg de PGF2α via intramuscular e 24 h após (dia 9 - D9) receberam 1,0 mg de BE via intramuscular. Foram realizadas avaliações ultrassonográficas com intervalos de 24 horas do D0 até o D8 e com intervalos de 12 horas da retirada do dispositivo de P4 até 96 horas. Para a dosagem de P4, foram colhidas amostras nos dias 0; 3; 6; 8; 10 e 22. A média do diâmetro máximo do folículo dominante (FD) foi menor (P=0,01) nas novilhas Gir (10,0 ± 0,8 mm) do que nas cruzadas Gir x HPB (13,0 ± 0,6 mm) ou HPB (12,5 ± 0,8 mm). Além disso, o tratamento com PGF2α no D0 aumentou (P=0,02) o diâmetro máximo do FD (12,9 ± 0,5 mm vs. 10,9 ± 0,7 mm) nos três grupamentos genéticos avaliados. A taxa de crescimento do FD foi menor (P=0,008) nas novilhas Gir (0,8 ± 0,1 mm/dia), do que nas novilhas cruzadas (1,3 ± 0,1 mm/dia) ou HPB (1,2 ± 0,1 mm/dia). As novilhas tratadas com PGF2α no D0 tiveram maiores (P=0,002) taxas de crescimento do FD (1,3 ± 0,1 vs. 0,9 ± 0,1 mm/dia) nos três grupamentos genéticos avaliados. A taxa de ovulação foi menor (P=0,02) nas novilhas Gir (36,4%) do que nas novilhas cruzadas (83,3%), mas não diferiu das novilhas HPB (70,0%). No entanto, as maiores (P<0,01) taxas de ovulação (93,7% vs. 35,3%) ocorreram nas novilhas tratadas com PGF2α no D0. A concentração circulante de P4 na retirada do dispositivo intravaginal (D8) foi maior (P<0,05) nas novilhas Gir (3,8 ± 2,2 ng/ml), do que nas novilhas cruzadas (2,2 ± 1,2 ng/ml), mas não foi observado contraste significativo nas novilhas HPB (2,4 ± 1,6 ng/ml). Durante todo o período de tratamento, as concentrações circulantes de P4 foram menores (P< 0,05) nas novilhas tratadas com PGF2α, do que aquelas que não receberam tratamento. As novilhas Gir apresentaram menor taxa de crescimento, menor diâmetro máximo do FD e uma tendência de menor taxa de ovulação, do que as novilhas HPB. O tratamento com PGF2α no dia da inserção do dispositivo intravaginal de P4 reduz a concentração circulante de P4, aumenta a taxa de crescimento e o diâmetro máximo do FD e a taxa de ovulação.


Palavras-chave


bovinos; dinâmica folicular; inseminação artificial; progesterona.

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Referências


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DOI: http://dx.doi.org/10.17523/bia.v74n2p122

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