Ononin promotes survival rate in cecal ligation puncture-induced sepsis rat model by regulating inflammatory pathway

Liu, BoWang, JingDuan, YingHussain, Shaik AlthafTripathi, Alok ShiomurtiLi, Kang

ABSTRACT Purpose: To investigate the potential protective effects of ononin on cecal ligation puncture (CLP) induced sepsis rat model. Methods: CLP was used to induce sepsis in rats and then treated with ononin at doses of 25 and 50 mg/kg intraperitoneally for seven days. The daily assessment included measurements of food and water intake, as well as body weight in the different rat groups. The study also examined the effects of ononin on survival rates, level of tumor necrosis factor (TNF)-α, interleukin (IL)-16, IL-6, C-reactive protein in the serum, and markers of oxidative stress in tissue homogenates of rats. The weight of spleen and lung tissue were also estimated in ononin-treated sepsis group. Additionally, histopathological examinations of lung and liver tissues were conducted in sepsis rats using hematoxylin and eosin staining. Results: The ononin-treated group showed significant improvements in food and water intake, as well as body weight, compared to the sepsis group of rats. Survival rate was also improved in the ononin-treated group. Ononin ameliorates oxidative stress and inflammatory mediators in sepsis rats. Histopathological changes (liver and lung tissue) were observed to be ameliorated in the group of rats treated with ononin versus the sepsis group. Conclusion: Ononin enhances survival rate in sepsis in rats by reducing inflammation and oxidative stress.