Effects of dexketoprofen trometamol on stress and oxidative stress in cattle undergoing claw trimming
Korkmaz, MusaSaritas, Zulfikar KadirDemirkan, IbrahimUlutas, Elmas
Background: Animal welfare raised a great deal of attention and associated with ethical and moral aspects. In animal,evaluation of wellness is a great tackle and has a complex entity. Pain and stress are closely associated with poor animalwelfare and animal suffering should be removed in order to achieve wellness. Today, good welfare in animal is definedas the absence of stress, thus most of the welfare studies focused on it. The aim of study was to investigate the effect ofdexketoprofen trometamol (DEXT) on stress and oxidative stress in cattle undergoing claw trimming.Materials, Methods & Results: This study was conducted on 14 female dairy cows aged 3 - 6 old years with mean bodyweight of 475 kg (7 Simmental, 5 Holstein Friesian and 2 Montafon). Each cow was randomly allocated into two groups.DEXT group (n = 8) was administered intravenously DEXT at a dose of 2 mg/kg body weight 30 min before claw trimming.Control group (n = 6) was administered physiological saline at the same dose 30 min before procedure was carried out.Trimming was performed using a mobile walk-in crush where the head of cow was restrained by a stanchion and the bodysupported thoracic and thigh belts in all procedures. The heart rate was measured by auscultation and respiratory rate bycounting thoracic excursions before and after claw trimming. Blood samples were collected by jugular venepuncture intoglass tube with gel, at 30 min before claw trimming and 15 and 30 min after trimming for measuring serum cortisol, nitricoxide (NO), malondialdehyde (MDA) and total antioxidant activity (AOA). Serum cortisol concentrations were determinedby ELISA with used commercial bovine ELISA kit. In both groups no difference was observed in heart rate however therespiratory rate at 15 and 30 min was significantly different (P < 0.05). The heart rate was increased at 15 min after clawtrimming...(AU)
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