Can we use LDL instead of egg yolk in BotuCrio® extender to cryopreserve sperm from the Mangalarga Marchador stallion?
Snoeck, Paola Pereira das NevesPessoa, Tainá Hortência OliveiraPereira, Marcelo Gonçalves SousaBastos, Inara Cristina LealMelo, Maria Isabel Vaz de
The objective of this study was to compare the BotuCrio® extender with the Merk - egg yolk and the INRA 82 modified by the inclusion of acetamide, methyl cellulose and trehalose in substitution of glycerol for freezing equine semen. The semen was diluted after centrifugation to obtain 100 x 106 of sperm/ml in: BotuCrio® (control); Merk - egg yolk or INRA 82 modified (Experiment 1). The extended semen was packaged in 0.5 ml straws, cooled and frozen in a freezing machine. The control extender was superior in preserving the motility, VCL, VSL, VAP, LIN, STR and the BCF when compared to the Merk - egg yolk and INRA 82 modified (P 0.05). The fertility rate after AI with frozen semen in BotuCrio LDL was 37.5%.
Texto completo