Ovarian structures modulate cellular aggregation and gene expression in oviductal isthmus cells

Pereira, Isabelle CristinaTeixeira, Ana Carolina BahiaWenceslau, Raphael RochaJorge, Erika CristinaRosa, Paola Maria da SilvaNogueira, Julia MeirelesCarvalho, Jade Carceroni de SousaPeruzzo, Gabriela PonathCarvalho, José de OliveiraOliveira, Leticia Zoccolaro

Abstract The presence of follicles and the corpus luteum (CL) in the ovarian surface plays a key role in determining the morphological and molecular fate of the female reproductive tract. However, the specific response of the isthmus epithelium to these ovarian structures remains poorly understood. This study hypothesizes that distinct ovarian structures differentially affect both the cellular aggregate-forming capacity of oviductal isthmus cells and the expression of ESR1, ESR2, and PGR genes. Reproductive tracts were categorized into three groups: ovaries with ≤5 mm follicles (small follicles group; SF); ovaries with follicles between 8-10 mm (large follicles group; LF); and ovaries containing active corpus luteum (CL Group). Isthmus cells from the three groups were cultivated to form cellular aggregates (oviductal explants) during 24 h. Moreover, the expression levels of ESR1, ESR2, and PGR genes were analyzed in the isthmus cells of the experimental groups. The isthmus cells of LF group showed an increased number of cellular aggregates than SF and CL group. Additionally, the SF group presented more aggregates than the CL group. Gene expression analysis revealed that ESR1 expression was higher in the SF group than in the LF group. Moreover, PGR expression was greater in the CL than in the SF group, as well as in the LF than in the SF group. In conclusion, ovarian structures impact the cellular aggregate formation capacity and the gene expression of ovarian steroid receptors in isthmus cells.