Standardization of sporulation and inoculation methodologies and reaction of acerola genotypes to dieback
Brandão, Leonardo AparecidoHonorato, Alan da CunhaSilva, Antonio Helton daCabral, Patrícia Gonçalves CastroSouza, Flávio de FrançaCapucho, Alexandre Sandri
ABSTRACT Acerola cultivation has been expanding in Brazilian Northeast region. However, diseases caused by Botryosphaeriaceae fungi, such as dieback, may affect acerola orchards. This study aimed to standardize sporulation and inoculation methods, to assess Lasiodiplodia sp. aggressiveness in three hosts, and resistance of acerola genotypes to dieback. Five substrates obtained from host species and five Botryosphaeriaceae isolates were used to induce sporulation, and three inoculation methods were assessed. The aggressiveness of eight Lasiodiplodia strains isolated from acerola was compared in mango, acerola, and grapevine seedlings and fruits. The resistance of 34 acerola genotypes was assessed after inoculation with an aggressive Lasiodiplodia strain. Sporulation was higher in the mango leaf and acerola branch, and the cork-borer method was the easiest and fastest one. Acerola was the most susceptible host, and there was a difference in the aggressiveness among Lasiodiplodia sp. strains. There was a difference in susceptibility to dieback among acerola genotypes. This study provides alternative methodologies for sporulation and inoculation of Botryosphaeriaceae fungi and resistant acerola genotypes to dieback for further use in breeding programs.
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