Immunoblot profiles of sera from laboratory rats naturally infected with Mycoplasma pulmonis and technicians exposed to infected animal facilities

Delgado, Márcio OliveiraTimenetsky, Jorge

Mycoplasma pulmonis have been isolated in about 10(5)CFU/mL from tracheal aspirates of rats from conventional animal facilities in São Paulo. The mycoplasma tranmission by aerosol may happen from an infected rat to a healthy one at distances up to 120 cm. This condition also favors the technicians contamination. As this infection is unknown, in humans, in this study the immunoblot profiles to M. pulmonis of sera from rats were compared to those presented by animal facility technicians. About 32 proteins from 11 to 230 kDa (kilodaltons) were recognized by the sera from rats naturally infected with M. pulmonis. Sera from technicians responsible for the cleaning and sanitation of cages of infected animals for more than seven years recognized about 10 proteins of this bacteria. Sera from individuals with shorter working time or that had never been exposed to such environment recognized few proteins. Proteins about 117 and 95 kDa were recognized by human and rat sera and by the negative controls. Although a positive human serm against M. pulmonis is unknown, this study stablished a temporary profile of protein recognition of human serum against such mycoplasma.