A reliable and efficient BioPulverizer method in preparing and grinding nematodes for nucleic acid extraction and molecular identification

Jiang, YeQin, RuifengLi, ChunjieHuang, MinghuiJiang, DanChang, DoudouXie, YifanZhao, YananWang, Congli

Due to their tiny size, soil nematodes and plant-parasitic nematodes are challenging to homogenize and collect using traditional mortar and pestle methods. To overcome this, we developed a reliable and efficient nematode preparation and grinding method using the BioPulverizer. The method involves creating a modified sample 'sandwich' with liquid nitrogen-frozen nematode samples placed between two layers of aluminum foil. This 'sandwich' facilitates complete homogenization through cryogenic grinding without any sample loss during transfer. The powerful nematode grinding yields high-quality and high-quantity samples, suitable for nucleic acid release and subsequent molecular identification and other downstream applications.

1. Homogenization
2. Soil nematodes
3. Cryogenic grinding
4. Plant parasitic